Coding

Part:BBa_K1523021:Design

Designed by: Haotian Wang   Group: iGEM14_LZU-China   (2014-10-03)


nahR coding sequence,encoding a LysR-type transcriptional regulator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 323
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 57
    Illegal PstI site found at 250
    Illegal PstI site found at 469
    Illegal NgoMIV site found at 491
    Illegal NgoMIV site found at 646
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

It's from the Pseudomonas putida NCIB 9816-4 plasmid pDTG1, complete sequence

References